Analysis of genetic diversity of wild Rehmannia glutinosa by using RAPD and ISSR markers / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2591-2595, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-324848
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the genetic diversity of wild Rehmannia glutinosa and evaluate and compare random amplified polymorphic DNA (RAPD) and inter sample sequence repeat (ISSR) for analysis of R. glutinosa accessions.</p><p><b>METHOD</b>Two molecular markers, RAPD and ISSR were used for analyzing 55 wild R. glutinosa accessions.</p><p><b>RESULT</b>Average 16.00 and 19.08 bands were amplified by RAPD primers and ISSR primers respectively, and the percentage of polymorphic bands were 89.58% and 94.32% respectively; Fifty-five R. glutinosa accessions categorized into 7 clusters were identified by unweighted pair-group method, arithmetic average (UPGMA) method.</p><p><b>CONCLUSION</b>A high level of genetic diversity of wild Rehmannia glutinosa was displayed at DNA level, and genetic diversity coefficient of R. glutinosa from different production areas was 0.63-0.98, and ISSR marker can detect higher genetic diversity of R. glutinosa germplasms than RAPD marker.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Variação Genética
/
Classificação
/
Técnica de Amplificação ao Acaso de DNA Polimórfico
/
Rehmannia
/
Genética
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2008
Tipo de documento:
Artigo
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