Development of bone marrow mesenchymal stem cell culture in vitro / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1650-1655, 2012.
Article
em En
| WPRIM
| ID: wpr-324920
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To review the in vitro development of bone marrow mesenchymal stem cells culture (BM-MSC).</p><p><b>DATA SOURCES</b>The data cited in this review were mainly obtained from articles listed in Medline and PubMed. The search terms were "bone marrow mesenchymal stem cell" and "cell culture".</p><p><b>STUDY SELECTION</b>Articles regarding the in vitro development of BM-MSCs culture, as well as the challenge of optimizing cell culture environment in two-dimensional (2D) vs. 3D.</p><p><b>RESULTS</b>Improving the culture conditions increases the proliferation and reduces the differentiation. Optimal values for many culture parameters remain to be identified. Expansion of BM-MSCs under defined conditions remains challenging, including the development of optimal culture conditions for BMSC and large-volume production systems.</p><p><b>CONCLUSIONS</b>Expansion of BM-MSCs under defined conditions remains challenges, including the development of optimal culture conditions for BMSC and scale-up to large-volume production systems. Optimal values for many culture parameters remain to be identified.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Células da Medula Óssea
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Células Cultivadas
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Técnicas de Cultura de Células
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Biologia Celular
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Células-Tronco Mesenquimais
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Métodos
Limite:
Animals
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Humans
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2012
Tipo de documento:
Article