Study on the response of specific antibodies against SARS-CoV in patients infected with SARS / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 856-858, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-325017
ABSTRACT
<p><b>OBJECTIVE</b>To study the response of specific antibodies against severe acute respiratory syndrome (SARS)-CoV in patients infected with SARS.</p><p><b>METHODS</b>IgM-capture, indirect and antigen-sandwiched enzyme linked immunosorbent assay (ELISA) were used to detect the SARS-CoV specific IgM, IgG and total antibodies in sera of clinical SARS patients or non-SARS individuals.</p><p><b>RESULTS</b>The positive rates of IgM, IgG and total antibodies to SARS-CoV in 146 sera of SARS patients collected in different phases of the disease were 61.64%, 53.43% and 69.86%, respectively. The earliest detectable days after onset of the disease for IgM and IgG to SRAS-CoV were 7 and 12 days, respectively. The specific IgM disappeared as early as 42 days after the onset of SARS. Of 70 sera from hepatitis A patients, 2 showed false positive results, while 127 sera from other patients were all negative, detected by the 3 methods. Serum from one medical worker who had been close contact to SARS patients was positive for anti-SARS-CoV IgG and total antibodies. These 3 methods used for detection were all not influenced by rheumatoid factor (RF).</p><p><b>CONCLUSION</b>All of the three methods were specific and sensitive for the detection of specific antibodies to SARS-CoV, and useful for epidemiological research and clinical diagnosis, but not for early diagnosis of SARS.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Sangue
/
Imunoglobulina G
/
Imunoglobulina M
/
Ensaio de Imunoadsorção Enzimática
/
Sensibilidade e Especificidade
/
Síndrome Respiratória Aguda Grave
/
Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
/
Alergia e Imunologia
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2004
Tipo de documento:
Artigo
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