A rapid colorimetric assay for viability of osteosarcoma cells after chemotherapy / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To introduce a rapid colorimetric method for assessing the viability of osteosarcoma cells after chemotherapy.</p><p><b>METHODS</b>Colorimetric assay and automatic microplate scanning spectrophotometer were used for assaying the viability of osteosarcoma cells.</p><p><b>RESULTS</b>Close correlation was found between the absorbance at 570 nm of the formazan products and the number of viable osteosarcoma cells.</p><p><b>CONCLUSION</b>An effective, sensitive and convenient colorimetric assay has been established to assess the survival of osteosarcoma cells following chemotherapy.</p>