Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2473-2476, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-325087
ABSTRACT
<p><b>OBJECTIVE</b>To establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs).</p><p><b>METHODS</b>The mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry.</p><p><b>RESULTS</b>The peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin production. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg).</p><p><b>CONCLUSION</b>Intraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Sangue
/
Imunização
/
Modelos Animais
/
Alergia e Imunologia
/
Eritrócitos
/
Imunidade Humoral
/
Cobaias
/
Proteínas Hemolisinas
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
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