siRNAs targetting sphingomyelin phosphodiesterase 1 protect mouse oocytes from apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 2165-2167, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-325157
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the potential of siRNAs targeting sphingomyelin phosphodiesterase 1 (SMPD1) in protecting the oocytes from apoptosis, and explore new approaches to female fertility preservation.</p><p><b>METHODS</b>Chemically synthesized siRNA targeting SMPD1 were introduced into mouse oocytes retrieved by hyperstimulation, and the cell apoptosis was analyzed by comic assay 48 and 72 h later.</p><p><b>RESULTS</b>In the oocytes without any siRNA injection, oocyte DNA damage occurred after 24 h, and large amount of DNA fragments migrated from the cells 48 h later. In oocytes injected with siRNA003, DNA migration decreased significantly as compared with the control and the other two groups injected with siRNA001 and siRNA002 (P<0.01).</p><p><b>CONCLUSION</b>siRNA targeting SMPD1 may protect the oocytes from apoptosis, and has the potential for use in future female fertility preservation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oócitos
/
Fisiologia
/
Esfingomielina Fosfodiesterase
/
Transfecção
/
Apoptose
/
Ensaio Cometa
/
Biologia Celular
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS