Effects of Rheb overexpression in HL-60 and K562 leukemia cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 268-272, 2013.
Article
em Zh
| WPRIM
| ID: wpr-325170
Biblioteca responsável:
WPRO
ABSTRACT
mTOR (mammalian target of rapamycin) is the center for cellular activities. It controls many cell activities via inhibiting apoptosis and promoting cell growth. Rheb can activate mTOR signaling pathway and participate in genesis and development of multiple cancers. This study was purposed to explore the underlying role of Rheb in human myeloid leukemia by using the myeloid leukemia cell lines. Two myeloid leukemia cell lines HL-60 and K562 overexpressing Rheb were established with retrovirus containing Rheb. The mRNA and protein expressions of Rheb were determined by Real-Time PCR and Western blot respectively. Cell proliferation rate was examined by CCK-8 assay and apoptosis rate was analyzed using Annexin V and 7-AAD double-staining. The results showed that Rheb was overexpressed in both HL-60 and K562 cell lines. The Rheb overexpression cell lines were successfully established. It is found that overexpression of Rheb could promote cell growth. Furthermore, the overexpression of Rheb could accelerate cells entering into G2/M phase (P < 0.01), while did not affect the apoptosis. It is concluded that Rheb overexpression promotes myeloid leukemia cell proliferation through accelerating cell cycle progression.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Neuropeptídeos
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Transdução de Sinais
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Ciclo Celular
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Células HL-60
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Células K562
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Proteínas Monoméricas de Ligação ao GTP
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Proliferação de Células
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Proteína Enriquecida em Homólogo de Ras do Encéfalo
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Metabolismo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2013
Tipo de documento:
Article