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Effect of gambogic acid on proliferation of SKM-1 cells and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 105-109, 2013.
Artigo em Chinês | WPRIM | ID: wpr-325203
ABSTRACT
The aim of this study was to explore the effect of gambogic acid (GA) on MDS SKM-1 cell proliferation, apoptosis and their possible mechanism. Cell proliferation was determined by MTT method. The apoptosis percentage and cell cycle regulation of SKM-1 cells were analyzed by flow cytometry. Morphological features were observed by light microscopy. The mRNA expression of bcl-2 and bax were detected by RT-PCR. The results showed that GA could inhibit the proliferation of SKM-1 cells in a dose- and time-dependent manner (IC50 was 0.37 µg/ml at 48 h), increase the apoptotic percentage of SKM-1 cells, and arrest cell cycle at the G0/G1. The expression of bax mRNA was up-regulated while that of bcl-2 mRNA was down-regulated in SKM-1 cells treated with GA for 48 h. It is concluded that GA can induce apoptosis, which may be related to its effect of arresting cells at phase of G0/G1 and down-regulating bcl-2/bax ratio.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Síndromes Mielodisplásicas / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Linhagem Celular Tumoral / Xantonas / Proliferação de Células / Proteína X Associada a bcl-2 / Pontos de Checagem do Ciclo Celular Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2013 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Síndromes Mielodisplásicas / Apoptose / Proteínas Proto-Oncogênicas c-bcl-2 / Linhagem Celular Tumoral / Xantonas / Proliferação de Células / Proteína X Associada a bcl-2 / Pontos de Checagem do Ciclo Celular Limite: Humanos Idioma: Chinês Revista: Journal of Experimental Hematology Ano de publicação: 2013 Tipo de documento: Artigo