Impact of olfactory nerve transection on the apoptosis of mice olfactory receptor neurons / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 671-674, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-325287
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the impact of olfactory nerve transection on the apoptosis of mice olfactory receptor neurons (ORN), and discuss the reliability of this experimental model.</p><p><b>METHODS</b>After olfactory nerve transection of mice, anterograde horseradish peroxidase (HRP) tracing was performed to confirm the completion of nerve transection. On 8 h, 2 d, 3 d and 5 d after surgery, TdT mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL) was used to observe the apoptosis of ORN, while relative semi-quantitative RT-PCR and immunohistochemistry were used to reflect the expression of olfactory marker protein (OMP, special marker of mature ORN) in olfactory epithelium.</p><p><b>RESULTS</b>No HRP label was observed in olfactory bulb after olfactory nerve transaction. Both TUNEL-positive and OMP-positive cells were ORN. After the surgery, TUNEL-positive cells increased remarkably and peaked on 2 d after the surgery. Meanwhile OMP mRNA in olfactory epithelium began to decrease markedly till 5 d after the surgery, and the olfactory epithelium got thinner accordingly.</p><p><b>CONCLUSIONS</b>This experimental model can be used reliably to sever mice olfactory nerve and manipulate simultaneous apoptosis of mice ORN.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Nervo Olfatório
/
Patologia
/
Cirurgia Geral
/
Apoptose
/
Neurônios Receptores Olfatórios
/
Biologia Celular
/
Denervação
/
Metabolismo
/
Camundongos Endogâmicos C57BL
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Ano de publicação:
2005
Tipo de documento:
Artigo
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