The targeting study of human serum albumin gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 223-228, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-325389
ABSTRACT
To generate transgenic porcine which expresses human serum albumin (HSA), the HSA gene targeting vector was constructed with HSA cDNA as the gene of interestand partial porcine serum albumin (PSA) gene as homologous arms which respectively were 7.2 kb 5' regulation sequence and 2.8 kb genomic sequence from the first intron to the fourth intron. The resistant gene neo was inserted into intron 1 and tk was ligated to the 3' end of the construct. Linearized targeting construct DNA was introduced into the fibroblast cells obtained from porcine fetus by electroporation. The positive-negative selection was performed and survival clones were screened by PCR and Southern blot. Three colonies with correct homologous recombination were obtained. Our results set a good basis for the establishment of transgenic porcine by gene target and nuclear transfer methods.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Albumina Sérica
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Transfecção
/
Sobrevivência Celular
/
Células Cultivadas
/
Southern Blotting
/
Reação em Cadeia da Polimerase
/
Clonagem Molecular
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS