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Detection of recombinant lysostaphin using antibody sandwish enzyme-linked immunoadsorbent assay / 生物工程学报
Chinese Journal of Biotechnology ; (12): 117-121, 2007.
Artigo em Chinês | WPRIM | ID: wpr-325408
ABSTRACT
The double-antibody-sandwich enzyme-linked immunoadsorbent assay (ELISA) for detection of rLysostaphin in humans had been developed and established through this study. rLysostaphin of high purity ( > 95 % ) produced in Shanghai Hi-Tech United Bio-Technological Research & Development Co., Ltd (SHUBRD) was used to produce a rabbit anti-rLysostaphin polyclonal antibody. The standard curve of rLysostaphin polyclonal antibody that was constructed showed that the lowest range of detection was found at 0. 98 ng of rLysostaphin/mL, and the curve exhibited linearity preferably from 0. 98 to 500 ng of rLysostaphin/mL. When three serum samples of the same batch were assayed for 6 replicates, and more 3 samples from different batches for 6 replicates, the average intra-assay and inter-assay coefficient variances ( CV) were 6. 4% and 6. 5%, respectively. The relative recovery rate was 98.6% when quantitative standard antigens were added to the serum. The present method for detection of rLysostaphin in serum is specific, highly sensitive, highly precise, and exhibited a low CV and will be helpful in the further study of rLysostaphin pharmacokinetics and holds promise in clinical applications.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Temperatura / Sangue / Estabilidade Enzimática / Proteínas Recombinantes / Ensaio de Imunoadsorção Enzimática / Western Blotting / Reprodutibilidade dos Testes / Alergia e Imunologia / Soros Imunes / Lisostafina Tipo de estudo: Estudo diagnóstico Limite: Animais / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Temperatura / Sangue / Estabilidade Enzimática / Proteínas Recombinantes / Ensaio de Imunoadsorção Enzimática / Western Blotting / Reprodutibilidade dos Testes / Alergia e Imunologia / Soros Imunes / Lisostafina Tipo de estudo: Estudo diagnóstico Limite: Animais / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2007 Tipo de documento: Artigo