Comparative study on in vitro stationary culture system or superfusion culture system of cow mammary tissue / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1040-1046, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-325428
ABSTRACT
Dairy cow mammary tissue was cultured in superfusion system or stationary system, and the influence of these two methods in the activity and ultrastructure of tissue was investigated according to LDH vigor, trypan blue dying, agrose gel electrophoresis, transmission microscope observation. The results showed that the mammary tissue cultured in superfusion system could keep normal tissue activity and ultrastructure within 12-60 h in DMEM plus 10% calf serum, while mammary tissue stationary culture could keep normal tissue activity and ultrastructure within 60-108 h. Both culture systems had some advantages and disadvantages.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Perfusão
/
Fatores de Tempo
/
Cinética
/
Microscopia Eletrônica
/
Reatores Biológicos
/
Técnicas de Cultura de Tecidos
/
Glândulas Mamárias Animais
/
Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Artigo
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