Study on monitoring and clearing of organophosphate in blood in organophosphate poisoned rats / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the new method of monitoring and clearing organophosphate in blood during single or mixed organophosphate(OP) poisoning.</p><p><b>METHOD</b>(1) Mixed equal volumes of blood of OP poisoned rat and healthy rat, then determine whole blood cholinesterase (ChE) activity. The descending range of ChE activity represents the level of residual OP in blood. (2) Poisoned rats by single or mixed OP pesticides were injected with 5% NaHCO3 15 ml/kg intraperitoneally, then the level of OP in blood was detected.</p><p><b>RESULTS</b>(1) The monitoring results of blood residual OP by gas chromatography were similar to that by "Mixes blood method", which showed significant difference(P < 0.05) from that before OP administration. (2) NaHCO3 injection could not improve the toxic symptoms and whole blood or brain ChE inhibition in 10 CP poisoned rats, blood residual OP level was also not affected, but lung pathological changes by OP such as interstitial inflammation and oedema showed some relief.</p><p><b>CONCLUSION</b>The monitoring of blood ChE by "mixed blood method" may reflect the general level of the blood residual OP within the range of exposure dose. The effect of NaHCO3 was not satisfactory, but it may improve OP-induced lung pathological changes.</p>