Study on immunogenicity of a recombinant adenovirus vaccine containing neuraminidase gene of H5N1 influenza virus (A/Anhui/1/2005) in mice / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 449-451, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-325515
ABSTRACT
<p><b>OBJECTIVE</b>To investigate immunity of a recombinant adenovirus vaccine (rAdV) containing codon-modified neuraminidase (Mod. NA) gene of H5N1 influenza virus in BALB/c mice and to screen for appropriate dose.</p><p><b>METHODS</b>BALB/c mice were immunized with the rAdV-Mod.NA vaccine intramuscularly twice (double injection at 0 and 4th week) in three groups, low dosage (10(5) TCID50 per dose), medium dosage (10(7) TCID50 per dose) and high dosage (10(9) TCID50 per dose). The effect of humoral and cell-mediated immunity were analysed at 5th week.</p><p><b>RESULTS</b>(1) The rAdV-Mod.NA vaccine could elicit both humoral and cell-mediated robust NA specific immunity in mice by neuraminidase inhibitor assay and IFN-gamma ELISpot assay; (2) 10(7) TCID50 per dose was the appropriate dose; (3) Peptide NA(109-124) CRTFFLTQGALLNDKH and peptide NA(182-199) AVAVLKYNGIITDTIKSW were the dominant epitopes for neuraminidase-immunized BALB/c mice, which was screened out from the whole length of neuraminidase of an H5N1 virus, A/Anhui/1l/2005.</p><p><b>CONCLUSION</b>The recombinant adenovirus NA could induce specific humoral and cellular immune responses in BALB/c after immunization, which suggest rAdV-Mod.NA vaccine was a potential vaccine candidate against H5N1 influenza and worthy of further investigation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Virais
/
Virologia
/
Vacinas contra Influenza
/
Vacinas Sintéticas
/
Distribuição Aleatória
/
Expressão Gênica
/
Adenoviridae
/
Relação Dose-Resposta Imunológica
/
Alergia e Imunologia
/
Influenza Humana
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2009
Tipo de documento:
Artigo
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