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Rescue of dendritic cells malfunction and loading with HBV proteins in patients with chronic hepatitis B in vitro / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 328-330, 2009.
Artigo em Chinês | WPRIM | ID: wpr-325552
ABSTRACT
<p><b>OBJECTIVE</b>To investigate dendritic cell (DC) malfunctions in patients with chronic hepatitis B (CHB) and try some means to restore the function in vitro.</p><p><b>METHODS</b>Twelve CHB patients and 10 healthy people were enrolled in the study. Phenotype analysis and allogeneic mixed lymphocyte reaction (AMLR) assay of DC from these subjects were made. Enzyme-linked ELISpot method for detecting IFN-gamma-producing CD8 (+) T cells were used to evaluate the efficacy of DC loaded in vitro with HBsAg or HBcAg.</p><p><b>RESULTS</b>DC from patients had a lower expression of co-stimulatory molecules and impaired AMLR capacity, but was restored partially by cytokine cocktail in vitro. Mature DC loaded with HBsAg or HBcAg showed a greater capacity for IFN-gamma-production than immature DC.</p><p><b>CONCLUSION</b>Malfunction of DC from CHB patients may be rescued by a cocktail of cytokines, and therapeutic DC vaccines loaded with HBV protein might be helpful to treat CHB patients.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Proteínas Virais / Virologia / Células Dendríticas / Estudos de Casos e Controles / Células Cultivadas / Vírus da Hepatite B / Citocinas / Hepatite B Crônica / Alergia e Imunologia Tipo de estudo: Estudo observacional Limite: Adulto / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2009 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Proteínas Virais / Virologia / Células Dendríticas / Estudos de Casos e Controles / Células Cultivadas / Vírus da Hepatite B / Citocinas / Hepatite B Crônica / Alergia e Imunologia Tipo de estudo: Estudo observacional Limite: Adulto / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Experimental and Clinical Virology Ano de publicação: 2009 Tipo de documento: Artigo