The expression of fibrillin 1 in pathologic scars and its significance / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 339-342, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-325847
ABSTRACT
<p><b>OBJECTIVE</b>To probe into the mechanism of fibrillin 1 in pathologic scar, by examining the expressions of fibrillin 1 and TGF-beta1 as well as their correlations in the tissues of keloid, hypertrophic scar and normal skin.</p><p><b>METHODS</b>The tissues of keloid, hypertrophic scar and normal skin were tested. RT-PCR was used to assess the mRNA expression levels of the aimed genes. The distribution of fibrillin 1 in scars and normal skin was examined by immunohistochemistry staining.</p><p><b>RESULTS</b>The mRNA level of fibrillin 1 in keloid (0.802 +/- 0.116) was increased by 218.25% (P < 0.01) than that in normal skin (0.252 +/- 0.067). The expression of the gene in hypertrophic scar (0.628 +/- 0.144) was higher by 149.21% (while, P > 0.05) than that in normal skin. The expression of TGF-beta1 in keloid and hypertrophic scar were more than that in normal skin. The expression of fibrillin 1 was related to that of TGF-beta1 positively (r = 0.820, P < 0.01). Fibrillin 1 protein was stained positively in basic membranes, endothelial cells, fibroblasts and extracellular matrix of skin tissues. In dermal, the protein levels of fibrillin 1 in keloid (0.117 +/- 0.042) was decreased than those in normal skin (0.185 +/- 0.043) and hypertrophic scar (0.181 +/- 0.048), the inhibition rates were 36.76%, 35.36% respectively (both P < 0.01).</p><p><b>CONCLUSIONS</b>The expression of fibrillin 1 in keloid was changed and related to the expression of TGF-beta1 positively, which appears that fibrillin 1 was a cicatrix specific gene. Fibrillin 1 might play an important role in the formation of keloid.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
RNA Mensageiro
/
Cicatriz Hipertrófica
/
Fator de Crescimento Transformador beta1
/
Fibrilinas
/
Fibrilina-1
/
Genética
/
Queloide
/
Metabolismo
/
Proteínas dos Microfilamentos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2008
Tipo de documento:
Artigo
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