Isolation, culture and characterization of endothelial cells in infantile hemangioma / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 144-147, 2008.
Article
em Zh
| WPRIM
| ID: wpr-325885
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the reliable isolation and culture of infantile hemangioma endothelial cells (HemECs) in vitro.</p><p><b>METHODS</b>Proliferative hemangioma specimens were digested by collagenase to form a single cell suspension. The HemECs were isolated using anti-CD31 coated dynabeads. The CD31+ cells were grown in fibronectin coated dishes. HemECs were identified by morphological characteristics and immunocytochemistry. The cells were also examined for their ability to intake LDL.</p><p><b>RESULTS</b>The method enabled the rapid isolation of HemECs that demonstrated typical endothelial cobblestone morphology in culture. The cells were positively stained for CD31, vWF. They also were labeled with DiI-Ac-LDL.</p><p><b>CONCLUSIONS</b>This technique can effectively isolate endothelial cells from the proliferative hemangiomas. These cells could be further used to research the mechanism of proliferation and degeneration of infantile hemangioma.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Hemangioma Capilar
/
Técnicas de Cultura de Células
/
Biologia Celular
/
Linhagem Celular Tumoral
/
Células Endoteliais
/
Citometria de Fluxo
/
Métodos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2008
Tipo de documento:
Article