Comparison of two nonalcoholic hepatocellular steatosis models / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare two models of nonalcoholic hepatocellular steatosis.</p><p><b>METHODS</b>HL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.</p><p><b>RESULTS</b>The results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.</p><p><b>CONCLUSION</b>A nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.</p>