Clinical significance of large-scale screening of A1555G mutation of mitochondria DNA for neonates / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 414-416, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-326921
ABSTRACT
<p><b>OBJECTIVE</b>To explore the necessity of large-scale screening of mitochondria DNA (mtDNA) A1555G mutation for prevention of aminoglycoside antibiotic induced deafness in newborns.</p><p><b>METHODS</b>One thousand blood filter samples were collected from neonates born in July 2008 in Shenzhen. DNA was extracted with Chelex-100 Resin and amplified by PCR. The mtDNA A1555G mutation was determined by denaturing high-performance liquid chromatography(DHPLC) for PCR products. The positive frequency was calculated.</p><p><b>RESULTS</b>The mitochondrial DNA A1555G mutation was detected in 2 cases of 1000 neonates. The frequency of mutation was 0.2%.</p><p><b>CONCLUSION</b>There is a high frequency of mtDNA A1555G mutation in neonates, the large-scale screening of mtDNAA1555G mutation in newborns might detect the individuals sensitive to aminoglycoside antibiotic, which is helpful to guide a rational medication for newborns and the maternal relatives at high-risk. Furthermore, it might be useful to prevent aminoglycoside antibiotic induced deafness.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
DNA Mitocondrial
/
Análise Mutacional de DNA
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Feminino
/
Humanos
/
Masculino
/
Recém-Nascido
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2011
Tipo de documento:
Artigo
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