Cytotoxicity of MICA-reactive V delta 1 gamma delta T cells towards epithelial tumor cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 1-7, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-326994
ABSTRACT
<p><b>OBJECTIVE</b>To confirm whether human MHC class I chain-related A (MICA) induces the amplification of V delta 1 gamma delta tumor-infiltrating lymphocytes (TILs) in vitro and to identify the cytotoxicity of MICA-reactive V delta 1 gamma delta TILs towards epithelial tumor cells.</p><p><b>METHODS</b>MICA protein was prokaryoticly expressed and purified by molecular cloning technology. The purified recombined MICA (rMICA) was used to induce V delta 1 gamma delta T cells from tumor tissues in vitro and the cytotoxicity of these V delta 1 gamma delta TILs were tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT).</p><p><b>RESULTS</b>The rMICA was expressed in prokaryocyte with pET30 as a vector. The immobilized rMICA protein could markedly induce the amplification of V delta 1 gamma delta T cells from tumor tissue in vitro. These V delta 1 gamma delta T cells showed strong cytolytic activities towards tumor cell lines expressing MICA.</p><p><b>CONCLUSION</b>The MICA-reactive V delta 1 gamma delta T cell may be a candidate for adoptive cellular therapy of tumors.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Ovarianas
/
Patologia
/
Proteínas Recombinantes
/
Células HeLa
/
Linfócitos T Citotóxicos
/
Antígenos de Histocompatibilidade Classe I
/
Imunoterapia Adotiva
/
Linfócitos do Interstício Tumoral
/
Receptores de Antígenos de Linfócitos T gama-delta
/
Alergia e Imunologia
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2004
Tipo de documento:
Artigo
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