Detecting HPV DNA in tissues of external genital squamous cell carcinoma in situ by PCR-RFLP technique / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 667-670, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-327012
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship between the infection of human papillomavirus (HPV) and external genital squamous cell carcinoma in situ.</p><p><b>METHODS</b>HPV DNA was detected with the consensus primer by polymerase chain reaction (PCR) and its type was determined by restriction fragment length polymorphism (RFLP).</p><p><b>RESULTS</b>HPV DNA was detected and HPV16 was the most frequently identified type in 29 (56.9%) of 51 cases of external genital squamous cell carcinoma in situ. HPV DNA was positive in 22 (55%) of 40 cases of bowenoid papulosis, among which 20 were of HPV16 type, one of HPV31 type, and one of HPV6 + 16 type. HPV DNA was positive in all 5 cases (100%) of Bowen's disease, among which 4 were HPV16 type and one of HPV6 + 16 type. HPV DNA was positive in 2 (33.3%) of 6 Queyrat erythroplasia cases and all were of HPV16 type.</p><p><b>CONCLUSION</b>HPV16 infection is strongly associated with the external genital squamous cell carcinoma in situ including bowenoid papulosis, Bowen's disease, and Queyrat erythroplasia.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Papillomaviridae
/
Neoplasias Penianas
/
Infecções Tumorais por Vírus
/
Virologia
/
Neoplasias Vulvares
/
Polimorfismo de Fragmento de Restrição
/
Doença de Bowen
/
DNA Viral
/
Carcinoma in Situ
/
Carcinoma de Células Escamosas
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2003
Tipo de documento:
Artigo
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