The Effect of GM-CSF Supplementation in Culture Medium in the Human IVF Programs / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 161-168, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-32725
ABSTRACT
OBJECTIVE:
Granulocyte-macrophage colony stimulating factors known to be secreted in murine and human reproductive tract. The development of human, bovine and murine embryos could be promoted by addition of GM-CSF in culture medium. However, the pregnancy and implantation rate of embryos cultured in GM-CSF have not been evaluated. The aim of this study was to assess the effect of GM-CSF in embryo development, pregnancy and implantation rate.METHODS:
A total of 191 IVF cycles were divided into control and GM-CSF supplement group (control =96, GM-CSF=95). The embryos were cultured for three day with or without 2 ng/ml of recombinant human GM-CSF. The quality of embryo, developmental velocity, pregnancy and implantation rates were compared.RESULTS:
There was no difference in age, number of gonadotropin ampules used, number of oocytes and fertilization. The number of ICSI cycle was higher in GM-CSF group. In GM-CSF group, G-1 grade embryos were the highest in proportion (56.4%), while G-2 grade embryos were highest (44.3%) in control group. The developmental velocity of embryos were not different between GM-CSF and control group. The pregnancy and implantation rates were significantly higher in GM-CSF group than control (47.4% vs. 33.3%, 17.0% vs. 11.1% respectively).CONCLUSION:
By adding GM-CSF in culture medium, the quality of embryo, pregnancy and implantation rate could be improved.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oócitos
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Fatores Estimuladores de Colônias
/
Injeções de Esperma Intracitoplásmicas
/
Desenvolvimento Embrionário
/
Estruturas Embrionárias
/
Fertilização
/
Gonadotropinas
Limite:
Feminino
/
Humanos
/
Gravidez
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Ano de publicação:
2001
Tipo de documento:
Artigo
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