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Effects of the site and method of liposuction on differentiation of human preadipocytes / 中华整形外科杂志
Chinese Journal of Plastic Surgery ; (6): 94-97, 2004.
Artigo em Chinês | WPRIM | ID: wpr-327302
ABSTRACT
<p><b>OBJECTIVE</b>To illustrate the effects of the site and method of liposuction on differentiation of human preadipocytes.</p><p><b>METHODS</b>Forty-two fatty samples were obtained with liposuction, which were then divided into four groups according to operation sites (abdomen, hip or extremity) and the methods (conventional negative-pressure or syringe method). Each sample was treated with collagenase I to release preadipocytes for in vitro culture. Affected by the differentiation-induced agents for 9 days or 28 days, the cultured adipocytes were stained with Nile red and observed under a fluorescent microscope. The differentiation rates were examined with flow cytometric analysis and the quantity of intracytoplasmic lipids was determined with oil red O staining. The results were analyzed with independent samples t-test (Mann-Whitney) using SPSS 10.0.</p><p><b>RESULTS</b>There was no statistical difference in differentiation at the 9th day or 28th day among the preadipocytes obtained from the abdomen, hip or extremity with the negative-pressure method. The preadipocytes from the abdomen differentiated more at the 28th day than the 9th day (P < 0.05), which was not observed in the hip or the extremity. The preadipocytes obtained from the abdomen with the negative-pressure method differentiated more than those with the syringe method (P < 0.05).</p><p><b>CONCLUSION</b>No essential difference was found in preadipocyte differentiation among the liposuction sites, while the abdomen might have some superiority. The negative-pressure method of liposuction is the first option in future research of tissue engineering. The flow cytometric analysis is a convenient way to study preadipocyte differentiation.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxazinas / Cirurgia Geral / Lipectomia / Diferenciação Celular / Células Cultivadas / Adipócitos / Biologia Celular / Abdome / Citometria de Fluxo / Quadril Limite: Adulto / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Plastic Surgery Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Oxazinas / Cirurgia Geral / Lipectomia / Diferenciação Celular / Células Cultivadas / Adipócitos / Biologia Celular / Abdome / Citometria de Fluxo / Quadril Limite: Adulto / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Plastic Surgery Ano de publicação: 2004 Tipo de documento: Artigo