Effects of Heparin-Binding Epidermal Growth Factor on the Expression of MMP-9 and ATPase gamma-subunit mRNA in the Mouse Embryo / 대한불임학회지
Korean Journal of Fertility and Sterility
;
: 87-94, 2001.
Artigo
em Coreano
| WPRIM
| ID: wpr-32734
ABSTRACT
OBJECTIVES:
The purpose of this study was to evaluate effects of heparin-binding epidermal growth factor (HB-EGF) on the rate of blastocyst formation and hatching in the mouse embryos and the expression of matrix metalloproteinase-9 (MMP-9) and ATPase g-subunit mRNA.METHODS:
Late 2-cell mouse embryos was cultured for 72 hours in HTF medium containing with 1, 10, and 100 ng/ml HB-EGF. The mRNA expression level of MMP-9 and ATPase gamma-subunit was detected by reverse transcription-polymerase chain reaction (RT-PCR).RESULTS:
The rate of hatching was significantly higher (p<0.05) in group containing with 1 ng/ml HB-EGF than other groups. Also, the rate of hatched blastocyst was significantly higher (p<0.05) in 10 ng/ml. The mRNA expression level of MMP-9 mRNA was not shown any difference among groups, but ATPase g-subunit was higher than other groups.CONCLUSIONS:
Taken together these results suggest that HB-EGF has the positive effect to promote the blastocyst formation and hatching process and influences the blastocoel expansion by promoting the ATPase mRNA expression in the mouse embryos.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Blastocisto
/
RNA Mensageiro
/
Adenosina Trifosfatases
/
Metaloproteinase 9 da Matriz
/
Estruturas Embrionárias
/
Fator de Crescimento Epidérmico
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Fertility and Sterility
Ano de publicação:
2001
Tipo de documento:
Artigo
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