Study on possibility of inorganic nitrogen utilization in Amauroderma elmerianum by labeling method with stable isotope 15N

ABSTRACT

The Black mannentake Amauroderma elmerianum Murr. has been newly found and cultivated in §µ L¹t, ViÖt Nam on mixed substrates, supplemented with inorganic nitrogen (ammonium sulfate), traced with 15N (4% excess). The efficiency of utilization of inorganic N fertilizer is determined about 70%, while organic N derived form various components in substrates (branch and bean powder) is only 7%. The amount of N in inorganic fertilizer added is less than 15% of total N amount in the substrates. The application of N fertilizer in combination with organic components, rich in N in substrates for cultivation of Lingzhi fungi as a material medica with improved quality has been discussed, based on using newly suggested 15N tracer techniques