Effect of andrographolide derivative Yanhuning on in vivo Candida albicans biofilms in rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2924-2929, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-327866
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of andrographolide derivative Yanhuning (YHN) on Candida albicans biofilms in rats.</p><p><b>METHOD</b>The rat C. albicans biofilms subcutaneous catheter model was established by intraperitoneally injecting YHN (40, 20, 10, 5, 2.5 mg x kg (-1)), with the FLC (80 mg x kg(-1)) positive group as the control group. After 7 d, CFU counting and XTT assay were used to evaluate the effect of YHN on C. albicans biofllms in vivo. Scanning electron microscopy (SEM) was applied to observe the morphological changes in rat biofilms intervened by YHN. The real-time fluorescence quantification PCR was adopted to detect expressions of C. albicans adhesion-related genes, such as ALS1, ALS3, HWP1, EAP1 and MP65.</p><p><b>RESULT</b>The YHN group showed much less CFUs on catheter pieces and lower XTT metabolic activity than the blank group, with dosage dependence. SEM also showed that YHN could obviously decrease C. albicans adhesion on subcutaneous catheters in rats. According to qRT-PCR's results, YHN can down-regulate expressions of ALS1, ALS3, HWP1, EAP1 and MP65.</p><p><b>CONCLUSION</b>YHN could inhibit C. albicans biofilms in rats.</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Candida albicans
/
Adesão Celular
/
Química
/
Biofilmes
/
Biologia Celular
/
Diterpenos
/
Relação Dose-Resposta a Droga
/
Catéteres
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2014
Tipo de documento:
Artigo
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