The effect of protein kinase B on the expression and location of p21 in early development of mouse fertilized eggs / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 493-496, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-327998
ABSTRACT
To investigate the effect of Protein kinase B on the expression and location of p21 in mouse early development. Immunopreciptation technology was used to detect the localization of p21 and Western blotting was used to analyze the expression of p21 after microinjecting mRNA of WT-PKB, myt-PKB and PKB-KD to mouse eggs. There was no obvious difference between the three kinds of mRNA in the expression of p21. But the cell localization altered. The p21 retain in cytoplasm after microjecting myt-PKB. In mouse fertilized egg PKB/Akt controls the cell cycle by changing the cell localization of p21.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Tempo
/
Zigoto
/
RNA Mensageiro
/
Núcleo Celular
/
Imunofluorescência
/
Biologia Celular
/
Citoplasma
/
Proteínas Proto-Oncogênicas c-akt
/
Inibidor de Quinase Dependente de Ciclina p21
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Artigo
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