Cloning and expression of a new glucoamylase gene / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 477-524, 2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-328001
ABSTRACT
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pichia
/
Rhizopus
/
Proteínas Recombinantes
/
Proteínas Fúngicas
/
Dados de Sequência Molecular
/
Regulação Enzimológica da Expressão Gênica
/
Regulação Fúngica da Expressão Gênica
/
Western Blotting
/
Glucana 1,4-alfa-Glucosidase
/
Clonagem Molecular
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2007
Tipo de documento:
Artigo
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