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Ad-ING4 inhibits K562 cell growth / 中华血液学杂志
Chinese Journal of Hematology ; (12): 396-400, 2007.
Artigo em Chinês | WPRIM | ID: wpr-328333
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of recombinant adenovirus Ad-ING4 on K562 cells.</p><p><b>METHODS</b>Human ING4 recombinant transfer vector pAdTrack-CMV-ING4 was constructed by enzyme digest and ligation of human ING4 gene which was obtained through site specific point mutation of mouse ING4. The vector was co-transduced into BJ5183 E. coli with pAdEasy-1. The new recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-hING4 was transfected into QBI-293A cells. To obtain the ING4 recombined adenovirus (Ad-ING4). Ad-ING4 was used to infect K562 cells. The effect on K562 cells of ING4 was tested by LSCM FCM and immunohistochemistry.</p><p><b>RESULTS</b>Human ING4 recombinant adenovirus vector was constructed successfully, and high titre ING4 recombinant adenovirus (Ad-ING4) was obtained. ING4 can down-regulate the expression of bcl-2 and up-regulate expression of bax. The apoptosis of K562 cells induced by ING4 was proved by LSCM FCM and immunohistochemistry. The apoptosis rate was 19.7% (after 72h), which displayed significant difference compared with that of control groups (P < 0.01).</p><p><b>CONCLUSION</b>Ad-ING4 can inhibit the growth of K562 cells and induce the cells apoptosis. The human ING4 recombinant adenoviral vector constructed might provide an approach to the target therapy of tumors.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Transformação Bacteriana / Dados de Sequência Molecular / Sequência de Bases / Transfecção / Proteínas de Transporte / Adenoviridae / Mutagênese Sítio-Dirigida / Apoptose / Proteínas de Homeodomínio Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2007 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Plasmídeos / Transformação Bacteriana / Dados de Sequência Molecular / Sequência de Bases / Transfecção / Proteínas de Transporte / Adenoviridae / Mutagênese Sítio-Dirigida / Apoptose / Proteínas de Homeodomínio Limite: Animais / Humanos Idioma: Chinês Revista: Chinese Journal of Hematology Ano de publicação: 2007 Tipo de documento: Artigo