Antiviral effects of aqueous extract from Spatholobus suberectus Dunn. against coxsackievirus B3 in mice / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 764-769, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-328475
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the antiviral effects of the aqueous extract of Spatholobus suberectus Dunn. (A.E.), a Chinese medicinal herb, against coxsackievirus B3 (CVB3).</p><p><b>METHODS</b>The antiviral effects of A.E. against CVB3 in vitro (primarily cultured myocardial cells) and in vivo (BALB/c mice) were determined. Serum pharmacological method was also adopted by in vitro experiments. The effects of A.E. inhibiting the CVB3 mRNA expression were compared by RT-PCR in mice in vivo.</p><p><b>RESULTS</b>A.E. exhibited obvious antiviral effects in vivo, and serum samples obtained from the rats with oral administration of A.E. (10 μg/mL, 5 μg/mL), reduced the virus titers in the infected myocardial cells (3.00±0.70, 3.55±0.52, P<0.01). Meanwhile, the viral myocarditis induced by CVB3 was inhibited significantly by A.E., and the 15-day mortality was reduced to 40% and 45% (P<0.01) in mice treated with A.E. at doses of 50 mg/kg and 100 mg/kg, respectively, while the 30-day mortality was decreased to 45% and 50%, respectively (P<0.01). Moreover, the mRNA expression of Coxsackie virus B3 was significantly inhibited by A.E.</p><p><b>CONCLUSION</b>Aqueous extract of Spatholobus suberectus Dunn. (A.E.) has inhibitory effect on CVB3 both in vitro and in vivo.</p>
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antivirais
/
Tamanho do Órgão
/
Patologia
/
Farmacologia
/
Virologia
/
Sangue
/
Peso Corporal
/
Células Vero
/
RNA Mensageiro
/
Extratos Vegetais
Limite:
Animais
Idioma:
Inglês
Revista:
Chinese journal of integrative medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
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