The effect of focal-adhesion micromanipulation in trauma and wound healing / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 57-60, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-328732
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.</p><p><b>METHODS</b>Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed.</p><p><b>RESULTS</b>After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinbeta1, alpha5 and tensin was dramatically reduced.</p><p><b>CONCLUSIONS</b>The morphology and the biological behaviour of the fibroblasts in hypertrophic scar can be changed after micromanipulation of focal adhesion.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cirurgia Geral
/
Cicatrização
/
Imuno-Histoquímica
/
Células Cultivadas
/
Cicatriz
/
Técnicas de Cultura de Células
/
Derme
/
Adesões Focais
/
Biologia Celular
/
Processos de Crescimento Celular
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2009
Tipo de documento:
Artigo
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