Strategy for the identification of amplified genes in tumors / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 474-478, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-328846
ABSTRACT
Amplification of genomic DNA is often observed in tumors. The identification of genes in amplified regions may be helpful to the discovery of oncogenes associated with a specific tumor. Array-based comparative genomic hybridization and restriction landmark genomic scanning are applicable to the definition of such regions. The copy number alterations of target genes present in these regions and the levels of their mRNA and protein can be characterized by quantitative PCR and tissue microarray staining with immunohistochemistry. Transfection, RNA interference, cDNA microarray or reverse transcription-multiplex ligation-dependent probe amplification will facilitate demonstrating the role of such amplified genes in the pathogenesis of a specific tumor.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Amplificação de Genes
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Interferência de RNA
/
Genética
/
Métodos
/
Neoplasias
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Artigo
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