A study of quadriplex PCR with chimeric primers for short tandem repeats loci / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 463-465, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-328849
ABSTRACT
<p><b>OBJECTIVE</b>To establish a simplified method of multiplex PCR based on chimeric primers for STR loci.</p><p><b>METHODS</b>A set of chimeric primers and universal primers were designed to carry out a multiplex PCR for amplifying four short tandem repeats(STR) loci. Then the amplified STR loci were detected by polyacrylamide gel electrophoresis and the gels were sliverstained.</p><p><b>RESULTS</b>A quadriplex STR system was developed on the basis of both chimeric and universal primers.</p><p><b>CONCLUSION</b>The STR multiplex PCR based on both chimeric and universal primers hss been achieved readily and reproducibly by simple adjustment of the individual primer concentrations. The use of chimeric primers provides a method for primer design that eliminates the multiple optimization steps involved in developing the multiplex PCR.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Primers do DNA
/
Repetições de Microssatélites
/
Genética
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Artigo
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