Rapid diagnosis of 21 trisomy syndrome by fluorescence quantitative polymerase chain reaction / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 398-399, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-328866
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of fluorescence quantitative PCR to detect 21 trisomy syndrome.</p><p><b>METHODS</b>At first, using one pair of primer to simultaneously amplify different fragments of two highly homologous genes of the human liver-type phosphofructokinase located on chromosome 21 (PFKL-CH21) and the human muscle-type phosphofructokinase located on chromosome 1 (PFKM-CH1). Then, staining the PCR products of these homologous genes with SYBR Green I, comparing the fluorescence intensities of the bands after electrophoresis, and analyzing the data.</p><p><b>RESULTS</b>The relative fluorescence intensity ratios of PFKL-CH21/PFKM-CH1 in 21 trisomy syndrome and normal individuals were 1.58+/-0.17 (mean+/-SD) and 1.00+/-0.05 (mean+/-SD), respectively; the difference between the two groups was highly significant.</p><p><b>CONCLUSION</b>SYBR Green I fluorescence quantitative polymerase chain reaction is an acurate, rapid, safe and practical approach for the detection of 21 trisomy syndrome.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Sensibilidade e Especificidade
/
Síndrome de Down
/
Diagnóstico
/
Fluorescência
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Criança
/
Criança, pré-escolar
/
Feminino
/
Humanos
/
Lactente
/
Masculino
/
Recém-Nascido
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Artigo
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