Detection of fetal SRY gene in maternal plasma by real-time fluorescence quantitative PCR / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 386-388, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-328870
ABSTRACT
<p><b>OBJECTIVE</b>To isolate fetal DNA from maternal plasma and examine its fetal origin.</p><p><b>METHODS</b>Fetal DNA in maternal plasma was isolated from 150 samples in the first trimester and mid-trimester of pregnancy, respectively. Real-time fluorescence quantitative polymerase chain reaction PCR (FQ-PCR) was used to determine sex-determining region Y (SRY) gene on Y chromosome.</p><p><b>RESULTS</b>Eighty-two women in the first trimester and 90 women in the mid-trimester carried male fetuses,70 and 90 samples of them were positive, respectively. The mean concentrations were (58.82+/-20.90) copies/ml and (152.08+/-62.61) copies/ml. The results of FQ-PCR were negative in the women who carried female fetuses.</p><p><b>CONCLUSION</b>The results show that fetal SRY gene can be found at a time as early as 42 days of gestation in maternal plasma by the use of FQ-PCR. The number of fetal DNA increases with gestational age. The real-time FQ-PCR is of great value in the non-invasive prenatal diagnosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Trimestres da Gravidez
/
Sangue
/
DNA
/
Reação em Cadeia da Polimerase
/
Idade Gestacional
/
Proteína da Região Y Determinante do Sexo
/
Feto
/
Fluorescência
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Feminino
/
Humanos
/
Gravidez
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Artigo
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