Enrichment and functional characterization of cancer stem cells from squamous cell carcinoma of head and neck by suspension culture / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 17-22, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-329006
ABSTRACT
<p><b>OBJECTIVE</b>Initiation, growth, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC) have been related to the cancer stem cells (CSC) that can be identified by their aldehyde-dehydrogenase-isoform-1 (ALDH-1) activity. In this study, we try to prove that suspension culture can enrich ALDH-1 high expression cells within HNSCC cell lines and the enriched cells possess cancer stem cell properties.</p><p><b>METHODS</b>Cells from five HNSCC cell lines were cultured in ultra-low attachment plates in serum-free Quantum 263 medium supplemented with 10 ng/ml EGF and 10 ng/ml bFGF, and ALDH-1 expression level was evaluated by ALDEFLUOR assay. ALDH-1 high expression cells were separated by FACS sorting, and their phenotypical and functional properties were characterized.</p><p><b>RESULTS</b>Spheroids can be formed from all five HNSCC cell lines (UD-SCC1, UT-SCC22, UM-SCC11B, UT-SCC9 and UT-SCC24A) under anchorage independent culture condition. The proportion of ALDH1 high expression cells were highly increased in speroids derived cells (SDCs) compared with their monolayers (P < 0.05). The clones formed by ALDH1 high expression cells on average contained 197 (197 ± 47) cells compared with 33 (33 ± 16) cells in clones generated from ALDH1 low expression cells (P < 0.01). Single ALDH1 high expression cell could significantly better regenerate a spheroid (UT-SCC9 17.1%, UD-SCC119.3%), whereas under the same conditions single ALDH1 low expression cells regenerated only in one case a spheroid (P < 0.01). SDCs from all five tested cell lines also showed a significantly increased invasion capacity (P < 0.05). We also found that the mRNA levels of Oct-4, Sox2, and Nanog were all significantly increased in the SDC. The reactive oxygen species (ROS) levels in SDCs from UD-SCC1 and UT-SCC24A were significantly increased compared with their monolayer counterpart [(26.3 ± 4.9)% vs (8.6 ± 1.7)% and (72.1 ± 6.1)% vs (23.7 ± 7.5)%, P < 0.05)].</p><p><b>CONCLUSION</b>Cancer stem cells can be enriched by suspension culture, which may be of importance in investigation of their contribution to therapy resistance, tumor recurrence and metastasis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Carcinoma de Células Escamosas
/
Regulação Neoplásica da Expressão Gênica
/
Técnicas de Cultura de Células
/
Linhagem Celular Tumoral
/
Neoplasias de Cabeça e Pescoço
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2014
Tipo de documento:
Artigo
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