Study of rat osteoblasts transfected by transforming growth factor beta 1 gene / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
;
(6): 116-143, 2002.
Artigo
em Inglês
| WPRIM
| ID: wpr-329167
ABSTRACT
In order to investigate the effect of TGF beta 1 gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by 3H-TdR and MTT. Our results showed that TGF beta 1 gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGF beta 1 gene transfer could promote the expression of TGF beta 1 and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defects in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Osteoblastos
/
Proteínas Recombinantes
/
Transfecção
/
Divisão Celular
/
Células Cultivadas
/
Fator de Crescimento Transformador beta
/
Técnicas de Transferência de Genes
/
DNA Complementar
/
Biologia Celular
/
Engenharia Tecidual
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Ano de publicação:
2002
Tipo de documento:
Artigo
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