High glucose promotes vascular smooth muscle cell calcification by activating WNT signaling pathway / 南方医科大学学报
Journal of Southern Medical University
;
(12): 29-33, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-329179
ABSTRACT
<p><b>OBJECTIVE</b>To investigate whether high glucose-induced vascular calcification is associated with WNT signaling pathway.</p><p><b>METHODS</b>An in vitro model of human vascular smooth muscle cell (VSMC) calcification was induced by exposure of the cells to high glucose. The expressions of WNT signal molecules and bone-related proteins including Cbfa1, Osx, OCN and BMP2 were analyzed with qRT-PCR, and the cell calcification was assessed by alizarin red staining. The effect of Dkk1, a WNT signaling inhibitor, on high glucose-induced cell calcification was tested with alizarin red staining and calcium content analysis.</p><p><b>RESULTS</b>High glucose activated WNT signaling pathway in human VSMCs by up-regulating the expressions of WNT signal molecules including Wnt3a, Wnt7a, Fzd4 and Wisp1 mRNA by 1.86, 1.68, 2.1, and 2.3 folds, respectively, and by promoting the phosphorylation of β-catenin (2.70∓0.22, P<0.05), a key mediator of WNT signaling pathway. Inhibition of WNT signaling pathway by Dkk1 attenuated high glucose-induced VSMC calcification and down-regulated the expression of bone-related proteins Cbfa1, Osx, OCN, and BMP2 by (51∓9)%, (58∓11)%, (56∓10)%, and (62∓10)% (P<0.01).</p><p><b>CONCLUSION</b>WNT signaling pathway is involved in high glucose-induced VSMC calcification.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Regulação para Cima
/
Células Cultivadas
/
Química
/
Biologia Celular
/
Miócitos de Músculo Liso
/
Calcificação Vascular
/
Via de Sinalização Wnt
/
Glucose
/
Músculo Liso Vascular
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Artigo
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