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Effects of sevoflurane preconditioning on cardiomyocyte apoptosis and myocardial inflammation in rats with sepsis / 南方医科大学学报
Journal of Southern Medical University ; (12): 1680-1683, 2014.
Artigo em Chinês | WPRIM | ID: wpr-329222
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of preconditioning with different concentrations of sevoflurane on cariomyocyte apoptosis and myocardial inflammation in rats with sepsis and explore the possible mechanism of sevoflurane for myocardial protection.</p><p><b>METHODS</b>Forty adult male Sprague-Dawley rats were randomly divided into 4 groups (n=10), namely the control group, LPS group, low-concentration sevoflurane group and high-concentration sevoflurane group. Following sevoflurane pretreatment for 30 min and a washout period for 10 min, all the rats received intraperitoneal injection of LPS or normal saline (NS) and were sacrificed 12 h later to observe the myocardial histopathology. Apoptosis of the ardiomyocytes was detected with TUNEL assay, and enzyme-linked immunosorbent assay was used to detect serum cTnI level and myocardial TNF-α level.</p><p><b>RESULTS</b>Compared with the control group, the rats in the other 3 groups showed significantly increased serum cTnI level, myocardial TNF-α content, and apoptotic index of the cardiomyocytes (P<0.05). Compared with those in LPS group, serum cTnI level, myocardial TNF-α content, and apoptotic index of the cardiomyocytes were significantly decreased in the two sevoflurane preconditioning groups (P<0.05), and the effect was more obvious with a high dose of sevoflurane (P<0.05

CONCLUSION:

Sevoflurane preconditioning can concentration-dependently reduce LPS-induced myocardial injury in rats possibly by decreasing cardiomyocyte apoptosis and alleviating myocardial inflammations.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Sangue / Fator de Necrose Tumoral alfa / Ratos Sprague-Dawley / Apoptose / Sepse / Troponina I / Biologia Celular / Miócitos Cardíacos Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2014 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Sangue / Fator de Necrose Tumoral alfa / Ratos Sprague-Dawley / Apoptose / Sepse / Troponina I / Biologia Celular / Miócitos Cardíacos Limite: Animais Idioma: Chinês Revista: Journal of Southern Medical University Ano de publicação: 2014 Tipo de documento: Artigo