Preliminary screening of downstream proteins of Sox2 and role of Sox2 in colonic cancer cell migration and invasion / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1594-1600, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-329240
ABSTRACT
<p><b>OBJECTIVE</b>To screen the down-stream proteins of transcription factor Sox2 and explore the role of Sox2 in the proliferation and migration of colonic cancer cells in vitro.</p><p><b>METHODS</b>The cellular proteins were separated by SDS-PAGE electrophoresis and stained with Coomassie blue and amine plated silver. The differentially expressed proteins was identified by mass spectrometry and verified by QPCR and Western blotting. A cell counting kit-8 (CCK8) assay was performed to evaluate the cell proliferation, and the cell migration was assessed using Transwell assay.</p><p><b>RESULTS</b>S3a was identified by proteomics technology as a Sox2-downregulated protein while ENO1 and gama-actin the up-regulated proteins. QPCR and Western blotting analyses showed that overexpression of Sox2 significantly decreased the expression of S3a (P<0.005) and increased the expression of ENO1(P<0.05), but had no significant effect on gama-actin expression. Sox2 overexpression obviously promoted cell proliferation and migration (P<0.05), while inhibition of Sox2 produced contrary effects (P<0.05).</p><p><b>CONCLUSION</b>Sox2 negatively regulates S3a expression and positively regulates ENO1 expression to promot the proliferation and migration of colonic cancer cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Ribossômicas
/
Regulação para Baixo
/
Regulação para Cima
/
Movimento Celular
/
Neoplasias do Colo
/
Proteômica
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Fatores de Transcrição SOXB1
/
Metabolismo
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
/
Estudo de rastreamento
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo
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