Mouse whole mount RNA in situ hybridization: an effective technique for analyzing gene expression / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 47-51, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-329401
ABSTRACT
<p><b>OBJECTIVE</b>To set up a method of analyzing gene expression profile from mouse whole embryos.</p><p><b>METHODS</b>Mouse whole mount RNA in situ hybridization(WM-ISH) of E10.5-E14 embryos was carried out by using digoxigenin-labeled Runx1 and Runx3 RNA probes and their expression profile was observed by detecting the existence and status of corresponding mRNAs in the embryonic tissues.</p><p><b>RESULTS</b>Clear hybridization signals were observed in different tissues and organs hybridized by Runx1 or Runx3 RNA probe. Different probes and ages of embryos had need of their own optimal proteinase K treatment conditions.</p><p><b>CONCLUSION</b>Mouse whole mount RNA in situ hybridization is an effective method of analyzing gene expression. It is useful for revealing whole gene expression profile and has a great potentiality in the era of functional genomics. It provides an alternative method of studies on gene expression which is at least as good as LacZ staining and immunohistochemistry. The key factor of the success to mouse whole mount RNA in situ hybridization is whether the proteinase K treatment conditions are optimal or not.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Transcrição
/
RNA
/
RNA Mensageiro
/
Proteínas Proto-Oncogênicas
/
Sensibilidade e Especificidade
/
Hibridização In Situ
/
Regulação da Expressão Gênica no Desenvolvimento
/
Perfilação da Expressão Gênica
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Proteínas de Ligação a DNA
/
Embrião de Mamíferos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Artigo
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