Further study on heterogeneic basis of complement C8 beta deficiency / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 10-13, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-329410
ABSTRACT
<p><b>OBJECTIVE</b>In Caucasian population, the most common molecular basis for C8 beta deficiency s a single C to T transition in exon 9 of C8 beta gene resulting in a stop codon. In previous family studies, two individuals were identified with C8 beta complete deficiency and were found to be only heterozygous for this mutation. This study was conducted by the present authors in search of other possible causes for these two C8 beta deficient individuals.</p><p><b>METHODS</b>Using direct DNA sequence analysis of all exon-specific PCR products of the C8 beta gene from these two C8 beta deficient patients and their descendants.</p><p><b>RESULTS</b>Two other C to T transitions at base 298 and 388 in exon 3 were detected, which could also create a termination codon. The descendants from one of the deficient patients were also analysed for the mutations, and it could be demonstrated that the two C to T mutations in exons 9 and 3 are segregating independently.</p><p><b>CONCLUSION</b>These two mutations, which create a termination codon, are sufficient to explain the complete C8 beta deficiency in both patients.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Linhagem
/
Complemento C8
/
DNA
/
Análise Mutacional de DNA
/
Química
/
Saúde da Família
/
Mutação Puntual
/
Heterogeneidade Genética
/
Códon sem Sentido
/
Genética
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2004
Tipo de documento:
Artigo
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