Deoxyribonuclease I gene expression in systemic lupus erythematosus patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 477-481, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-329430
ABSTRACT
<p><b>OBJECTIVE</b>To observe whether deoxyribonuclease I (DNASE1) gene expression and its DNASE1 mRNA expression was detected by real-time polymerase chain reaction and its alternatively spliced transcripts were performed by capillary electrophoresis. An analysis was also made to disclose whether specific single nucleotide polymorphisms (SNPs) haplotype had effects onDNASE1 gene expression and its alternatively spliced transcripts.</p><p><b>RESULTS</b>DNASE1 gene expression was higher in SLE patients than in normal controls (P<0.001), and in patients it was found to be of no relationship with SLE disease activity index score. However, it was increased in female patients. Capillary electrophoresis revealed that the pattern of alternatively spliced transcripts in patients was not the same as that in normal controls. Moreover, it seemed that different SNPs haplotype combination might show different transcript pattern in SLE patients.</p><p><b>CONCLUSION</b>In SLE patients, DNASE1 gene expression is abnormal and there are alternatively spliced transcripts different from those in normal controls. DNASE1 gene is a critical factor in the pathogenesis of SLE.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Expressão Gênica
/
Reação em Cadeia da Polimerase
/
Processamento Alternativo
/
Polimorfismo de Nucleotídeo Único
/
Desoxirribonuclease I
/
Genética
/
Lúpus Eritematoso Sistêmico
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2003
Tipo de documento:
Artigo
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