8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage induced by environmental tobacco side-stream smoke and its mechanism / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 43-47, 2005.
Artigo
em Inglês
| WPRIM
| ID: wpr-329603
ABSTRACT
<p><b>OBJECTIVE</b>To study the genotoxicity effect of environmental tobacco side-stream smokes (ETSS) on oxidative DNA damage and its molecular mechanism.</p><p><b>METHODS</b>DNA adduct 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. The level of 8-OHdG in DNA exposed to ETSS was detected by high performance liquid chromatography with electrochemical detection. Organic and inorganic components in ETSS were analyzed by gas chromatography-mass spectrum and atomic absorption spectrum respectively.</p><p><b>RESULTS</b>Particle matters (PMs) and volatile organic compounds (VOCs) in ETSS could directly induce oxidative DNA damage and formation of 8-OHdG. There were 123 and 84 kinds of organic components in PMs and VOCs respectively, and 7 kinds of inorganic components in ETSS. Some components, especially quinones and polyphenols in ETSS, could produce free radicals in vitro by auto-oxidation without any biological activity systems, and with the catalytic reaction of metals, the DNA adduct 8-OHdG was produced.</p><p><b>CONCLUSION</b>ETSS have biological oxidative effect on DNA in vitro and in vivo, and expressed direct genotoxicity. 8-OHdG is a valuable biomarker of oxidative DNA damage.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Compostos Orgânicos
/
Oxirredução
/
Poluição por Fumaça de Tabaco
/
Dano ao DNA
/
DNA
/
Biomarcadores
/
Química
/
Adutos de DNA
/
Metais Pesados
/
Desoxiguanosina
Limite:
Animais
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2005
Tipo de documento:
Artigo
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