Breeding of transgenic mice expressing human tau isoform with P301L mutation and identification of homozygous transgenic mice / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 221-224, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-329903
ABSTRACT
<p><b>OBJECTIVE</b>To establish homozygous transgenic mouse strain expressing human tau isoform with P301L mutation.</p><p><b>METHODS</b>Five transgenic mice expressing human tau isoform with P301L mutation were obtained by microinjection into male nuclei. Homozygote and hemizygote were identified by PCR and real-time fluorescent quantitative PCR.</p><p><b>RESULTS</b>Ninety five homozygous transgenic mice were selected, and the results indicated that homozygous transgenic mice were superior to hemizygote in simulating the changes of biological characteristics.</p><p><b>CONCLUSION</b>Exogenous gene tau is able to stably transmit to next generation and the combination of SYBR Green real-time fluorescent quantitative PCR with the traditional mating is a fast, reliable and economical way to screen homozygous and hemizygous transgenic mice.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Camundongos Transgênicos
/
Proteínas tau
/
Genética
/
Homozigoto
/
Camundongos Endogâmicos C57BL
/
Microinjeções
/
Mutação
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2012
Tipo de documento:
Artigo
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