Recombinant human bone morphogenetic protein-2 transform NIH3T3 fibroblast to osteo-like cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To examine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on NIH3T3 cells.</p><p><b>METHODS</b>The NIH3T3 cells were cultured in DMEM with 50 microg/L rhBMP-2. The proliferation ability, alkaline phosphatase (ALP) activity and osteocalcin (OC) quantity of NIH3T3 cells were investigated.</p><p><b>RESULTS</b>The cells showed decreased proliferation ability but enhanced ALP activity and OC quantity.</p><p><b>CONCLUSION</b>50 microg/L rhBMP-2 can transform NIH3T3 fibroblasts to osteo-like cells.</p>