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Homocysteine' s effect on tPA and PAI-1 gene expression in HUVEC / 中国应用生理学杂志
Chinese Journal of Applied Physiology ; (6): 363-366, 2004.
Artigo em Chinês | WPRIM | ID: wpr-330094
ABSTRACT
<p><b>AIM</b>In order to elucidate the relationship between homocysteine (Hcy) and the fibrinolytic system, we examined the effect of Hcy on tissue- type plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) gene expression in human umbilical vein endothelial cells (HUVEC) in vitro.</p><p><b>METHODS</b>Total RNA was extracted from HUVEC exposed to physical and pathological concentrations of Hcy (0, 10, 50, 200, 500 micromol/L ) for 24 hours, using the guanidinium isothiocyanate method. The semi-quantification of tPA and PAI-1 mRNA in HUVEC was carried out by reverse transcriptase-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>PAI-1 mRNA levels was enhanced by Hcy at concentrations of 500 micromol/L of Hcy, compared with that of 0 micromol/L Hcy (P < 0.05). The mRNA expression of tPA, however, was significantly decreased at concentrations of 500 micromol/L Hcy, compared with that of 10 micromol/L Hcy (P < 0.05), but compared with the control group, the tPA level of 10 micromol/L Hcy was much higher (P < 0.05).</p><p><b>CONCLUSION</b>Hyperhomocysteinemia increases the incidence of cardio cerebral vascular disease, which may be caused by decreasing the activity of fibrinolytic system, whereas, the physiological concentration of Hcy may be decreased the incidence by enhancing the activity of fibrinolytic system.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / RNA Mensageiro / Expressão Gênica / Células Cultivadas / Ativador de Plasminogênio Tecidual / Inibidor 1 de Ativador de Plasminogênio / Células Endoteliais da Veia Umbilical Humana / Genética / Homocisteína / Metabolismo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2004 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / RNA Mensageiro / Expressão Gênica / Células Cultivadas / Ativador de Plasminogênio Tecidual / Inibidor 1 de Ativador de Plasminogênio / Células Endoteliais da Veia Umbilical Humana / Genética / Homocisteína / Metabolismo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Applied Physiology Ano de publicação: 2004 Tipo de documento: Artigo