Effect of ginkgolides on gene expression of HIF-1alpha in primary cultured neurons / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To study the effects of ginkgolides (Gin) on the expression of hypoxia inducible factor-1 (HIF-1alpha) in hypoxic/ischemic neurons.</p><p><b>METHODS</b>The gene expression of HIF-1alpha pretreated with or without Gin (37.5 microg/ml) was observed by RT-PCR on primary cultured cortical neurons in the condition of hypoxia and oxygen-glucose deprivation.</p><p><b>RESULTS</b>Some basic expression of HIF-1alpha mRNA were observed in cultured cortical neurons. The expression of HIF-1alpha mRNA increased after 24 h treatment with Gin. The level of HIF-la mRNA increased also after 1 h hypoxia and further enhanced after the pretreatment with Gin. The expression of HIF-1alpha mRNA decreased with the deprivation of both oxygen and glucose, which reversed after the pretreatment of Gin.</p><p><b>CONCLUSION</b>Gin could increase the expression of HIF-1alpha mRNA in hypoxic/ischemic cortical neurons.</p>