The role of p38 on the differentiation of MSCs to myoblasts / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 345-348, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-330099
ABSTRACT
<p><b>AIM</b>Inducing mesenchymal stem cells (MSCs) differentiate to myoblasts with 5-azacytidine(5-Aza-CR), investigating the expression of Myf5 and the role of the signal transduction case of p38 in all the course of differentiation.</p><p><b>METHODS</b>Separating and purifying bone marrow-derived MSCs, inducing MSCs differentiation to myoblasts with 10 micromol/L 5-Aza-CR, assaying the gene expression time of Myf5 with RT-PCR method, the antigen expression of myosin with immunohistochemistry method and observing the changes of the activity of phosphorylation p38 before and after inhibited by SB203580 with Western-blot method.</p><p><b>RESULTS</b>MSCs begin to express Myf5 delayed to the 9th day after inhibited by SB203580. Some of MSCs express myosin at the 7th day after induced; The phosphorylation p38 activity of MSCs enhanced after induced by 5-Aza-CR but obviously decreased after inhibited by SB203580.</p><p><b>CONCLUSION</b>MSCs can express myogenic regulator factors and orientation differentiate to myoblasts after induced by 5-Aza-CR, p38 really have a positive signal transduction affection in this course.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Azacitidina
/
Células da Medula Óssea
/
Transdução de Sinais
/
Diferenciação Celular
/
Células Cultivadas
/
Biologia Celular
/
Mioblastos
/
Proteínas Quinases p38 Ativadas por Mitógeno
/
Fator Regulador Miogênico 5
/
Células-Tronco Mesenquimais
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2004
Tipo de documento:
Artigo
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