Testosterone has beneficial effects on human umbilical vein endothelial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 338-341, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-330103
ABSTRACT
<p><b>AIM</b>To investigate the influences of testosterone with varied concentrations on the functions of HUVEC.</p><p><b>METHODS</b>Human umbilical vein endothelial cells within 2-3 passages were cultured with testosterone (3 x 10(-10) to 3 x 10(-8), 3 x 10(-6), 3 x 10(-5) mol/ L), and the control confluent cells were cultured in the same medium without steroid. MTT experiment was repeated for 7 days to investigate each groups' cell proliferation. The values of NO were tested as recommended. The tPA and PAI-1 antigen levels were assayed with ELISA Kits.</p><p><b>RESULTS</b>Testosterone at physiologic or lower concentrations (3 x 10(-10) to 3 x 10(-8) mol/L ) had no adverse effect on A490 and NO level, meanwhile, stimulated the secretion of tPA (P < 0.01). However, tPA levels markedly reduced at larger dose (3 x 10(-6) to 3 x 10(-5) mol/L). On the other hand, PAI-1 antigen levels decreased significantly at the testosterone concentrations ranging from 3 x 10(-10) to 3 x 10(-5) mol/L (P < 0.05).</p><p><b>CONCLUSION</b>Testosterone at physiologically relevant concentrations affectively decreased PAI-1, while increased tPA levels, which suggested that testosterone might have beneficial effects on the Human umbilical vein endothelial cells and cardiovascular system to prevent atherosclerosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Testosterona
/
Células Cultivadas
/
Ativador de Plasminogênio Tecidual
/
Inibidor 1 de Ativador de Plasminogênio
/
Células Endoteliais da Veia Umbilical Humana
/
Metabolismo
/
Óxido Nítrico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2004
Tipo de documento:
Artigo
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